Carrier Z6 Manuale Utente

Updated July 2013Written by Gracen BrilmyerLEICA APPLICATION SUITE V 4.2 IMAGING MANUALThe Field Museum of Natural History — Moreau Lab (www.moreaulab

2. BEFORE YOU BEGINA Log on to computer using FM\ log-inB If you don’t already have one, create a new user folder in E:\User Important: All informati

3. INITIAL SETUPA Open LAS V4.2 1 Confirm Hardware Setup: [TAB] Setup → [SECTION] Microscope Configuration 4. ACQUIRING IMAGESA Prepare specimen Pi

D Adjustments - [TAB] Acquire → [SUBTAB] Camera → [SECTION] Exposure Adjust 1 If using, pull the Dome Illuminator to the “down” position 2 Set the s

2 [TAB] Acquire → [SUBTAB] Z → [SECTION] Options a Check the following boxes (if not already checked): Create Multifocus after stack acquire

Background: Out of FocusBackground: NoneB [TAB] Process → [SUBTAB] Z → [SECTION] Montage Enhance Choose options in this section as needed (see Suggest

Note: At any step in the processing, go to [TAB] Process → [SUBTAB] Z → [SECTION] Undo/Redo to step back or forward in the editing process. Keep in mi

B Montage Enhancing — [TAB] Process → [SUBTAB] Enhance → [SECTION] Levels 1 Minor adjustments can be made in this section, such as increasing black

B Delete the individual images - [TAB] Browse → [SUBTAB] Browse [SECTION] Navigator — since the final montage has been exported, the individual images

APPENDIX

A. SPECIMEN POSITIONINGThe following are standard imaging positions for insect specimens (particularly ants). All specimens are imaged using a 60% gra

The Moreau Lab would like to thank Dr. Brian Fisher and AntWeb.org for their helpful advice as well as for sharing their imaging manual and equipment

3 Head The clypeus and top of the head should be in the same plane of focus. If eyes are present, the same amount of each eye should visible. When

B. CHOOSING LIGHTING1 For most specimens, the Dome Illuminator should work great: Illuminates specimen from all angles, and can focus lighting on eith

C. EXPOSURE (OPTIONAL)To check if any parts of your specimen are under- or over-exposed go to:[TAB] Acquire → [SUBTAB] Camera → [SECTION] DFC450 - Las

D. WHITE BALANCE (OPTIONAL)1 Once your lighting is set, place a white sheet of paper on the microscope stage. Click the “Automatic white balance on wh

E. STANDARD SETTINGS1 Hardware Setup: [TAB] Setup → [SECTION] Microscope Configuration Optics Carrier Icon - should see used Optics Carrier used liste

F. NAMING PROTOCOLS1 Ants: FMNHINS1234567_(image view)_Genus_sp_(type status)_(additional shot number).tif Final file name for images of ants should i

o Olympus Q Color OQCcom – Olympus Q Color camera attached to Olympus compound scope OQCste - Olympus Q Color camera attached to Olym

G. SCALE BAR SETTINGSThe standard scale bar setting for Insect images are as follows: Mode: User Length Style: Horizontal End Bar: None Size: 0.1m

H. EXACT EQUIPMENTAPPENDIXComputerShuttle PC Computer Econ Version 1080p LED full DH Monitors (x2)Camera & ColumnVideo Objective 0.63xNo : 1044736

First time logging in to computer iQuick Workflow (for experienced users) ii1 Equipment 1 A Parts B Tools C How to change the Objective D H

GETTING STARTED

FIRST TIME LOGGING IN TO COMPUTERIf you are a brand new user to this computer, you will need to follow the following steps to ensure the Leica Applica

1 Log on to computer using FM\ log-in2 Open user folder on E:\User3 Choose and Optics Carrier and lens (confirm in Hardware Configuration)4 Open LAS V4.

IMAGING

1. EQUIPMENTA Leica DFC450 Camera with Video Objective 0.63xB Optics Carrier (Z6 or Z16) C Iris D ZoomE Lens (Objectives: 0.5x, 1.0x, and 2.0x)F Go

HOW TO CHANGE LENSES (0.5x, 1.0x, 2.0x)1 If Dome Light is attached, first remove it (see How to change Dome Illuminator below)2 Make sure not to touch